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. 2010 Dec 10;107(52):22546–22551. doi: 10.1073/pnas.1012310108

Fig. 4.

Fig. 4.

Stabilization of hVDAC1 by the E73V mutation and by DCCD reaction. (A) Ratio of peak intensities between E73V and WT hVDAC1 observed in 1H,15N-spectra, averaged over a three-residue window. The orange dashed line indicates the threshold value of 1.6. Residues with a larger ratio were mapped onto the three-dimensional structure (PDB code 2JK4) in orange (inset). Residues that, in contrast to E73V hVDAC1, could not be assigned in WT hVDAC1 are mapped in red. (B) Top: chemical exchange rates (Rex) of E73V (black bars) compared to WT values from Fig. 1B (red area). Difference values (E73V— WT) are shown below. (C) DCCD-induced changes in resonance intensities observed in 1H,15N-TROSY spectra of hVDAC1, averaged over a three-residue window. Purple circles indicate residues that show up after DCCD treatment. The orange dashed line is the threshold value of 1.2. (A–C) The topology of hVDAC1 is indicated at the top, showing the location of the N-terminal helix and the β-strands. Residues that, in contrast to E73V hVDAC1, could not be assigned in WT hVDAC1 are highlighted in red, and a blue diamond marks the position of E73.