Fig. 3.

Complex formation is inhibited by PHD domain. (A) EMSA of proteins containing noncore regions with 5 nM radiolabeled 12RSS and 5 nM 23RSS. The concentrations of RAG proteins were varied (40, 20, 10, 5, 2.5 nM of each, indicated left to right). The 12/23RSS PC and the single substrate complex (SC) are indicated. These reactions contained no HMGB1. (B) EMSA of constructs containing noncore regions with 10 nM precleaved substrates (12SE-cy5 and 23SE). The concentrations of RAG1/RAG2 were each 50, 25, and 12.5 nM estimated as a heterotetramer.