Figure 4.

[³H]LTD₄ binding to membrane proteins from CHO cells expressing the mouse CysLT₁ receptor. (A) Saturation analysis of [³H]LTD₄ binding to CHO cell membranes from transformants expressing an isoform of the mouse CysLT₁ receptor (■, short isoform; ▴, long isoform; ●, negative control). (Insets) Scatchard plot analyses of the short isoform (Left) and the long isoform (Right) are also shown. (B) Competition for [³H]LTD₄ binding to CHO cell membranes expressing the short isoform (Left) and the long isoform (Right) of the mouse CysLT₁ receptor (■, LTD₄; ●, LTC₄; ▴, LTE₄; ⧫, LTB₄). Results are representative of at least three independent analyses.