Fig. 4.

Insulin action is decreased in STAT6-null mice. (A and B) Impaired glucose homeostasis in STAT6-null mice fed a HFD. Wild-type (dashed line) and STAT6-null (solid line) mice (8–10 wk old) were fed HFD for 8 to 10 wk (n = 5 per genotype). Increased glucose intolerance (A) and insulin resistance (B), as assessed by intraperitoneal glucose tolerance test (2 g/kg) and insulin-tolerance test (0.75 U/kg). (C) Serum insulin levels were quantified by ELISA. (D) Decreased insulin signaling in STAT6^−/− mice, as assessed by quantification of phospho-AKT. (E) Increased hepatic triglycerides in STAT6^−/− mice (n = 5). (F) Induction of lipogenic gene expression in livers of STAT6^−/− mice. All transcripts were measured by qRT-PCR (n = 5). Data presented as mean ± SEM. *P < 0.05; **P < 0.01.