Table 2.
Kinetic and thermodynamic parameters for the folding and unfolding kinetics of the Im7 variants
| KUI | kIN(s-1) | kNI(s-1) |  |
 |
|
| Variant |
(MUI [kJ·mol-1·M-1]) |
(mIN [kJ·mol-1·M-1]) |
(mNI [kJ·mol-1·M-1]) |
kJ·mol-1 |
kJ·mol-1 |
| WT Im7 | 125.2 ± 30 (3.9 ± 0.1) | 276 ± 13 (0.74 ± 0.10) | 1.31 ± 0.13 (0.51 ± 0.04) | −11.4 ± 0.6 | −23.9 ± 0.6 |
| A29C N5 | 302 ± 58 (3.66 ± 0.08) | 151 ± 3 (0.54 ± 0.04) | 1.09 ± 0.11 (0.49 ± 0.03) | −13.4 ± 0.5 | −25.0 ± 0.5 |
| A29C N5 Glyco | 352 ± 66 (3.70 ± 0.07) | 207 ± 4.5 (0.69 ± 0.04) | 1.07 ± 0.11 (0.46 ± 0.03) | −13.8 ± 0.4 | −26.2 ± 0.5 |
| A29C A13N | 253 ± 103 (3.86 ± 0.17) | 197 ± 10 (0.85 ± 0.10) | 1.08 ± 0.15 (0.60 ± 0.05) | −13.0 ± 1.0 | −25.3 ± 1.0 |
| A29C A13N Glyco | 218 ± 91 (3.92 ± 0.18) | 178 ± 9 (0.86 ± 0.11) | 1.38 ± 0.17 (0.52 ± 0.04) | −12.7 ± 1.0 | −24.1 ± 1.0 |
| A29C K20N | 67.6 ± 12.7 (3.96 ± 0.07) | 191 ± 7 (0.81 ± 0.10) | 1.43 ± 0.08 (0.49 ± 0.02) | −9.9 ± 0.4 | −21.4 ± 0.5 |
| A29C K20N Glyco | 14.7 ± 8.6 (4.43 ± 0.11) | 169 ± 11 (0.47 ± 0.23) | 2.58 ± 0.08 (0.51 ± 0.01) | −6.3 ± 0.7 | −16.2 ± 0.68 |
| A29C V27N | 67.9 ± 7.8 (4.09 ± 0.05) | 166 ± 4 (0.56 ± 0.07) | 1.33 ± 0.05 (0.52 ± 0.01) | −9.9 ± 0.3 | −21.3 ± 0.3 |
| A29C V27N Glyco | 432 ± 93 (4.35 ± 0.10) | 140 ± 4 (0.57 ± 0.05) | 1.43 ± 0.13 (0.37 ± 0.03) | −14.3 ± 0.5 | −25.1 ± 0.6 |
| D59C N60 | 267 ± 35 (4.13 ± 0.06) | 159 ± 3 (0.64 ± 0.04) | 1.55 ± 0.09 (0.37 ± 0.02) | −13.2 ± 0.3 | −24.1 ± 0.3 |
| D59C N60 Glyco | 296 ± 51 (4.14 ± 0.07) | 199 ± 5 (0.79 ± 0.05) | 1.25 ± 0.09 (0.42 ± 0.02) | −13.4 ± 0.4 | −25.3 ± 0.4 |
| D59C K73N | 112 ± 16 (3.82 ± 0.06) | 134 ± 2 (0.70 ± 0.05) | 2.50 ± 0.10 (0.42 ± 0.01) | −11.1 ± 0.3 | −20.4 ± 0.4 |
| D59C K73N Glyco | 15.8 ± 3.9 (3.77 ± 0.16) | 132 ± 8 (0.92 ± 0.34) | 2.61 ± 0.10 (0.43 ± 0.01) | −6.5 ± 0.7 | −15.7 ± 0.7 |
| D59C A78N | 92.2 ± 8.5 (4.06 ± 0.04) | 170 ± 3 (0.66 ± 0.05) | 1.62 ± 0.05 (0.40 ± 0.01) | −10.6 ± 0.2 | −21.6 ± 0.23 |
| D59C A78N Glyco | 63.7 ± 8.9 (4.05 ± 0.06) | 133 ± 4 (0.42 ± 0.08) | 1.28 ± 0.07 (0.46 ± 0.02) | −9.8 ± 0.3 | −20.7 ± 0.4 |
All rate constants are expressed in s-1, all m values are expressed in kJ·mol-1·M-1 and all free energies are expressed in kJ·mol-1. Data were acquired at 10 °C in 50 mM sodium phosphate (pH 7.0) and 0.4 M Na2SO4. Errors were calculated as described previously (19).