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Impact of MTX concentration and pH on the activity of PCFT D109 mutants. (A) [3H]-MTX influx (10μM) over 1 minute at pH 5.5. (B) [3H]-MTX influx (100μM) at pH 5.5. (C) [3H]-MTX influx (0.5μM) at pH 4.5. (D) [3H]-MTX influx (100μM) at pH 4.5.
