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. 2010 Nov 18;10:253. doi: 10.1186/1471-2229-10-253

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Copyright ©2010 Ahn et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Subcellular localization of O. sativa immunophilins using GFP fusion proteins. The full length ORF of Os immunophilins FKBP15-2, FKBP20-1a, FKBP20-1b, CYP19-2 and CYP20-2 were fused to the N-terminal mGFP5 in pCAMBIA1302 and expressed in N. benthamiana leaf cell transformed using Agrobacterium. Localization of fluorescent signals was examined at 48 h after Agrobacterium infiltration under fluorescence microscopy. The images of GFP, chlorophyll auto-fluorescence and bright-field images are shown.