Table 2.
Conservation of key residues for Cyclosporin A binding/PPIase activity in O. sativa cyclophilins (OsCYPs) and comparison with those of A. thaliana cyclophilins.
| OsCYPs/AtCYPs | Residues for Cyclosporin A binding/PPIase activity in human cyclophilinA (aa position) |
Conserveda (%) |
Similarityb (%) |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 54 | 55 | 60 | 111 | 113 | 121 | 126 | ||||
| hCYPA | H | R | F | Q | F | W | H | |||
| OsCYP17 | S | Q | I | " | " | " | Y | 43/- | ||
| OsCYP18-1 | : AtCYP18-1 | " | " | " | " | " | H | Y | 71/71 | 100 |
| OsCYP18-2 | : AtCYP18-2 | " | " | " | " | " | S | " | 86/86 | 100 |
| : AtCYP18-3 | " | " | " | " | " | " | " | -/100 | ||
| OsCYP18-4 | : AtCYP18-4 | " | " | " | " | " | " | " | -/100 | |
| : AtCYP19-1 | " | " | " | " | " | " | " | -/100 | ||
| OsCYP19-2 | : AtCYP19-2 | " | " | " | " | " | " | " | 100/100 | 100 |
| OsCYP19-3 | : AtCYP19-3 | " | " | " | " | " | " | " | 100/100 | 100 |
| OsCYP19-4 | : AtCYP19-4 | " | " | " | " | " | R/" | " | 88/100 | 88 |
| OsCYP20-1 | : AtCYP20-1 | " | " | " | " | " | " | " | 100/100 | 100 |
| OsCYP20-2 | : AtCYP20-2 | " | " | " | " | " | " | " | 100/100 | 100 |
| OsCYP20-3 | : AtCYP20-3 | " | " | " | " | " | " | " | 100/100 | 100 |
| OsCYP21-1 | : AtCYP21-1 | " | " | " | " | " | " | " | 100/100 | 100 |
| : AtCYP21-2 | " | " | " | " | " | " | " | -/100 | ||
| : AtCYP21-3 | S | " | K | E | " | D | L | -/29 | ||
| OsCYP21-4 | : AtCYP21-4 | R/Q | H/" | F/Y | D/E | L | D | L | 0/14 | 43 |
| OsCYP22 | : AtCYP22 | " | " | " | " | " | " | " | 100/100 | 100 |
| OsCYP23 | : AtCYP23 | F | " | " | S | S | H | Y | 29/29 | 100 |
| : AtCYP26-1 | D | H | L | " | O | Q | " | -/29 | ||
| OsCYP26-2 | : AtCYP26-2 | V | K | Y | E | V | E | A/V | 0/0 | 86 |
| OsCYP28 | : AtCYP28 | " | K | Q | E | L | E/Q | N | 14/0 | 86 |
| OsCYP37 | : AtCYP37 | Y | T | L | " | " | F | F | 29/29 | 100 |
| OsCYP38 | : AtCYP38 | F | " | " | " | " | I | Y | 57/57 | 100 |
| OsCYP40a | : AtCYP40 | " | " | I | " | " | H | " | 71/71 | 100 |
| OsCYP40b | : AtCYP40 | " | " | I | " | " | H | " | 71/71 | 100 |
| OsCYP57 | : AtCYP57 | " | " | " | " | " | " | N/" | 86/100 | 86 |
| OsCYP59a | : AtCYP59 | R/Q | K | Q | " | Y | Y | " | 29/29 | 86 |
| OsCYP59b | : AtCYP59 | R/Q | K | Q | " | Y | Y | " | 29/29 | 86 |
| OsCYP63 | : AtCYP63 | " | F/" | M/" | " | " | H | " | 57/86 | 71 |
| OsCYP65 | : AtCYP65 | " | " | " | " | " | H | " | 86/86 | 100 |
| OsCYP71 | : AtCYP71 | " | " | " | " | " | " | " | 100/100 | 100 |
| OsCYP95 | : AtCYP95 | " | " | " | "/H | "/H | R/Q | S/N | 57/43 | 43 |
| Conservedc (%) | 71/77 | 79/79 | 72/69 | 82/79 | 71/71 | 38/52 | 62/69 | |||
a% identity of rice/Arabidopsis CYP compared to human cyclophilin A. b% similarity between rice and Arabidopsis CYP. C% conservation of amino acid in rice/Arabidopsis CYP