Table 3. Expression of selected genes of retinal progenitor cell (mRPC) in epidermal growth factor (EGF) and EGF+ Glial cell line-derived neurotrophic factor (GDNF) conditions.
| Genes | Description | Microarray | RT–PCR |
|---|---|---|---|
| Ki-67 | Cell cycle protein, proliferation marker | N | Upregulation (fold change 1.07) |
| Nestin | Intermediate filament | N | N |
| Sox2 | Transcription factor | N | N |
| Hes1 | Transcription factor | N | N |
| Hes5 | Progenitor marker | N | Upregulation (fold change 1.03) |
| Notch1 | Progenitor marker | Down regulation# (fold change 0.98) | N |
| Pax6 | Paired box | N | N |
| CXCR4 | chemokine (C-X-C motif) receptor 4 | N | N |
| DCX | Doublecortin,neuroblast marker | N | N |
| Vimentin | Intermediate filament | N | Upregulation (fold change 1.20) |
| GFAP | Intermediate filament | Upregulation (fold change 1.10) | N |
| PKC-α | Bipolar neurons marker | N | N |
| Recoverin | Phototransduction-related protein | N | N |
| Rhodopsin | Rod photoreceptors marker | N | N |
| β3-tubulin | Microtubule protein | N | N |
| Caspase 1 | Apoptosis marker | N | N |
| Caspase 3 | Apoptosis marker | N | N |
| Annexin A5 | Apoptosis marker | N | N |
| KLF4 | Kruppel-like factor 4, induced pluripotent stem cells marker | N | N |
| Nanog | Nanog homeobox | Upregulation** (fold change 1.05) | N* |
| P4HB | prolyl 4-hydroxylase, beta polypeptide, Fibroblast Marker | N | N |
| GDNF | glial cell line derived neurotrophic factor | N | N |
* N: No statistically significant difference was found between EGF and EGF+GDNF conditions. ** Upregulation: The expression of genes was upregulated in EGF+GDNF comparing EGF. # Down regulation: The expression of genes was down regulated in EGF+GDNF comparing EGF.