Figure 3.

Enhancement of HIV-1 'tat' mediated transactivation by rhesus MSS-1. Aliquots of the TZM-bl cell line were transfected with either 0.2 μg of HIV-1 'tat' expression plasmid alone, 0.8 μg of MSS-1 expression plasmid alone, or both and dispensed into individual wells of a 96-well microtiter plate (5000 cells/well) in media for 48 hr. Each assay was performed in triplicate. B-galactosidase activity was then determined using the Tropix Gal-screen assay kit and the results expressed as mean RLU/sec. The data shown are representative of 3 separate experiments. The S.D. of the 3 cultures was all < 10%.