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. 2010 Dec 30;5(12):e14397. doi: 10.1371/journal.pone.0014397

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Plews et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) OCT4-RFP localizes into nucleus in fibroblast cells. (B) FACS analysis of OCT4 protein expression 24 hrs after mRNA microporation. Cy3 conjugated secondary antibody was used. (C)Western blot showing corresponding protein expression in 10⁶ MRC5 cells transfected with OCT4 (17 µg), SOX2 (10 µg), cMYC (6 µg), KLF4 (6.5 µg), SV40LT (3.5 µg). The negative control is GFP mRNA transfected MRC5 cells. HUES1 is human ES cells.