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. 2010 Dec 30;5(12):e14457. doi: 10.1371/journal.pone.0014457

Figure 2. Morphology and identification of bFGF over-expressing hFLSCs feeder cells.

Figure 2

(A): eGFP expression of γ-irradiated bFGF-hFLSCs imaged by fluorescence microscope. Bars: 100 µm. (B): Western blotting analysis indicated that bFGF highly expressed in γ-irradiated bFGF-hFLSCs. (lane 1) γ-irradiated hFLSCs transfected with control vectors; (lane 2) γ-irradiated bFGF- hFLSCs; (lane 3) A half amount of the lane 2. (C): The enzyme-linked immunosorbent assay (ELISA) to bFGF showed that bFGF-hFLSCs could stably secret bFGF for one week. Values are presented as means ± SD, n = 4.