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. 2001 Jan 23;98(5):2268–2273. doi: 10.1073/pnas.031488298

Figure 4.

Figure 4

The E. coli initiator tRNA mutant is aminoacylated in E. coli only in the presence of the yeast TyrRS mutants, and the aminoacyl-tRNA is active in suppression. (A) Growth of E. coli CA274 transformed with pACamG (carrying the mutant suppressor tRNA gene and the CATam27 gene), and pKK223-3 (plus and minus the mutant TyrRS genes) on a 2× YT plate containing 100 μg/ml chloramphenicol. (B) Acid–urea gel analysis of the mutant tRNA expressed in E. coli in the presence and absence of the TyrRS mutants.