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. 2010 Dec 30;5(12):e15689. doi: 10.1371/journal.pone.0015689

Figure 2. Phenotype of UCB stromal/MSC during long-term culture as analyzed by flow cytometry and immunocytochemistry.

Figure 2

Flow cytometric analysis of UCB-derived stromal/MSC cultivated in MesenCult medium with added supplements and 5 ng/ml FGF-β. (A) FSC/SSC plot of MSC at P7. Gate for live cells is indicated. (B) No expression of the haematopoietic markers CD34, CD45 or CD14 on UCB-derived MSC (open histograms). The filled histogram represents the isotype control. (C) Expression of the stromal/MSC markers CD73, CD44, CD29 and CD105 (open histograms). The filled histograms represent isotype controls. (D) FSC/SSC plot of MSC. Sub-confluent vs confluent cultures at P24. Gates for live cells are indicated. (E) Expression of MSC markers (open histograms) at P24 by cells phenotyped at sub-confluent vs. confluent cultures. The filled histograms represent isotype controls. (F) Immunocytochemistry of stromal/MSC prepared from P7 and from confluent culture at P24. Positive peroxidase staining was seen with Nestin and CD133 but not with CD45. (G) Shows cell scatter and phenotype of stromal/MSC obtained from selected CD34+ cells at P3. Stromal/MSC were positive for CD73, CD29, CD105 and CD44 (open histograms) and negative for haematopoietic cell markers CD34, CD45 and CD14. The filled histograms represent isotype controls.