Skip to main content
. 2010 Dec 30;5(12):e15689. doi: 10.1371/journal.pone.0015689

Figure 5. CFU-F colonies of MSC and surface expression of various mesenchymal stem cell markers expressed by MSC generated from steady state PB.

Figure 5

Stromal/adherent MSC could be generated from steady state peripheral blood (obtained from 8/8 healthy donors) during the expansion of haematopoietic stem cell in stroma-free liquid culture at day 6 (A, left panel). A confluent growth of stromal adherent cells was obtained at day 28 (A, right panel) (scale bar: 200 µm). (B) A CFU-F colony generated from PB stromal/MSC after P1 (day 28) (scale bar: 100 µm). (C) Flow cytometric analysis of PB-derived stromal/MSC at d28 were positive for stromal/MSC markers CD29, CD105, CD73, CD44 and negative for haematopoietic markers CD45, CD34, CD14. The filled histograms represent isotype controls, respectively.