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. 2010 Dec 30;5(12):e15888. doi: 10.1371/journal.pone.0015888

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Choi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Double reciprocal plots of the initial velocity of ApHMW1C as a function of UDP-glucose concentration at the fixed His-HMW1ct concentrations, as indicated (inset). (B) Double reciprocal plots of the initial velocity of ApHMW1C as a function of His-HMW1ct concentration at the fixed UDP-glucose concentrations, as indicated (inset). The true K_m values of UDP-glucose and His-HMW1ct corresponded to 54.5 µM and 2.3 µM, respectively, as obtained by eq. 2.