Table 1. Kinetic parameters of ApHMWC and its derivative proteins.
| Parameter a | Km | Vmax | kcat | kcat/Km |
| (µM) | (nmol min−1 mg−1) | (s−1) | (M−1s−1) | |
| UDP-Glc | ||||
| ApHMW1C | 38.9±5.8 | 108.8±4.0 | 0.130±0.005 | (3.3±0.8) ×103 |
| P55 | 67.3±3.8 | 141.0±2.4 | 0.132±0.002 | (2.0±0.6) ×103 |
| P15 | ND | ND | ND | ND |
| UDP-Gal | ||||
| ApHMW1C | 147.5±24.8 | 64.3±4.3 | 0.077±0.005 | (5.2±2.1) ×102 |
| P55 | 206.8±50.8 | 44.2±4.9 | 0.041±0.005 | (2.0±0.9) ×102 |
| P15 | ND | ND | ND | ND |
a
These are apparent values, determined by varying the concentration of one substrate (sugar donor substrate) at a fixed concentration of the second (protein acceptor).