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. 2010 Dec 30;5(12):e16000. doi: 10.1371/journal.pone.0016000

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Bohr et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Analysis of purification of recombinant CCN2/CTGF from supernatants of stable transfected WB4 clone by SDS-PAGE and subsequent Coomassie stain. Bacterially expressed CCN2/CTGF (1 and 2 µg) served as an internal control. (B) Western blot analysis of individual fractions using a CCN2/CTGF-specific antibody. (C) Analysis of purification of CCN3/NOV from supernatants of COS-7 that were transiently infected with Ad-CMV-CCN3/NOV in Ponceau S stain. (D) The membrane from (C) was probed with a CCN3/NOV-specific antibody. (E) Different amounts (20 and 45 µg) of purified CCN3/NOV taken from fraction 2 (C, D) were analysed in Coomassie stain (left panel), Ponceau S stain (middle panel) and Western blot (right panel) with a CCN3/NOV-specific antibody.