Figure 3. Size-exclusion chromatography of purified CCN proteins.

(A) Size-exclusion gel chromatography of fraction 2 (cf. Figures 2C and 2D) and subsequent detection of CCN3/NOV in Western Blot. (B) Size-exclusion gel chromatography of bacterial expressed recombinant CCN2/CTGF (BioVendor) in a buffer containing 10 mM Tris (pH 7.0). (C) Size-exclusion gel chromatography of purified recombinant human CCN2/CTGF in a buffer containing 10 mM Tris (pH 7.0). (D) Size-exclusion gel chromatography of purified recombinant human CCN2/CTGF in a buffer containing 10 mM Tris (pH 7.0) and 0.15 M NaCl. As gel filtration standard in (B–D) a mixture of molecular weight markers containing Vitamin B₁₂ (1.35 kDa), horse Myoglobin (17 kDa), chicken Ovalbumin (44 kDa), bovine γ-Globulin (158 kDa), and bovine Thyroglobulin (670 kDa) was applied.