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1 µg of purified CCN2/CTGF and CCN3/NOV that were kept for three months at indicated temperatures (4°C, −80°C) were separated on a NuPAGE Bis/Tris 4–12% gradient SDS gel under reducing and non-reducing conditions and stained with Coomassie. In this analysis 1 µg of rhCTGF (BioVendor) that was stored at −80°C served as an internal control.
