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. 2010 Dec 30;5(12):e16000. doi: 10.1371/journal.pone.0016000

Figure 7. Biological activity of purified recombinant CCN2/CTGF, part I.

Figure 7

(A) EA•hy 926 cells in serum-free growth medium were incubated with indicated concentrations of bacterial expressed recombinant CCN2/CTGF (BioVendor) and subjected to BrdU incorporation assay. (B) EA•hy 926 cells in serum-free growth medium were subjected to indicated concentration of purified recombinant CCN2/CTGF and analysed in BrdU incorporation assay. The observed proliferation rates were set in relation to control cells that received no recombinant CCN2/CTGF. (C) EA•hy 926 cells in serum-free growth medium were left untreated or stimulated with TGF-β1 (1 ng/ml) and PDGF-BB (25 ng/ml) and the proliferation was measured in BrdU assay. (D) EA•hy 926 cells were transiently transfected with the (CAGA)12-Luc reporter plasmid and incubated with TGF-β1 (10 ng/ml), indicated concentrations of recombinant CCN2/CTGF or left unstimulated. The relative luciferase activity was measured after 24 hr. The gene promoter activity of the unstimulated control cells was set to 1 in this experiment.