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. 2010 Dec 30;5(12):e16000. doi: 10.1371/journal.pone.0016000

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Bohr et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) 1 µg of recombinant CCN3/NOV or CCN2/CTGF was subjected to deglycosylation assay using the endoglycosidase Endo H (removes only high mannose and some hybrid types of N-linked carbohydrates) and PNGase F (removes carbohydrates attached to an Asn). The digested proteins were then resolved by SDS-PAGE (NuPAGE Bis/Tris 4–12% gradient) and probed with indicated antibodies.