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Subclonfluent, proliferating Caco-2 cells were transfected with Mybl2-specific or control, nontargeting (NT) siRNA for 48 hours. RNA (A) and protein (B) were measured as described in Materials and Methods. In (A), bar represents the average + standard deviation of 3 independent experiments, each performed in duplicate. (C) RNA from Mock- or Mybl2 siRNA-treated cells was measured for expression of A-myb, c-myb, and Mybl2 by qRT-PCR as described in Materials and Methods.
