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. 2010 Oct 15;96(1):69–77. doi: 10.3324/haematol.2010.026997

Figure 1.

Figure 1.

RAD001 synergizes with radiation in a dose-dependent manner to increase ALL cell death. (A) Representative flow cytometry dot plots showing the analysis used to determine cell viability. The viability of the LK63 cells in the examples provided is shown in the lower right corner of each dot plot. ALL cells negative for annexin V and 7-AAD were considered viable. (B) LK63 cells were treated with the indicated concentrations of RAD001 for 24 h with or without exposure to 5 Gy of ionizing radiation and analyzed as in (A). Between two and six replicates were examined at each point and the mean and standard deviation are shown. (C) Data obtained in (B) were square root transformed. Divergent lines represent agonistic, parallel lines additive, and convergent lines antagonistic relationships. (D) NALM6, REH and LK63 cells were treated with 16 μM RAD001 and/or the indicated dose of ionizing radiation or 40 ng/mL of dexamethasone. The mean and standard deviation of triplicate determinations are shown. *P<0.05 compared to 16 μM RAD001 or ionizing radiation indicating an agonistic interaction.