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. 2001 Feb 13;98(5):2274–2277. doi: 10.1073/pnas.051598298

Figure 1.

Figure 1

Nanoelectrospray ionization tandem mass spectrometry (nanoESI-MS/MS) experiment performed on a modified RNA nucleotide. FHV RNA was extracted from N-acetyl-aziridine-treated and -untreated virus and exposed to RNase. The nanoESI-MS/MS results show that the treated virus had covalent N-acetyl-aziridine modification on approximately 0.4% of the nucleotides. The mass spectrometry analyses were performed on a Perkin–Elmer Sciex API III (Sciex, Thornhill, ON, Canada) modified with a nanoESI source from Protana A/S (Denmark). Palladium-coated, boro-silicate glass capillaries from Protana A/S were used for nanoelectrospray sample delivery.