Figure 5.

Localization of SsgA and SsgB during different stages of development in S. coelicolor. (Left to right) SsgA-eGFP (green), SsgB-mCherry (red), an overlay, and a bright-field image. The strain that was used is S. coelicolor JSC4 (Supplemental Table S3). SsgA localizes prior to SsgB in young aerial hyphae (0.6 μm wide) (top row), and the two proteins colocalize only briefly during an early stage of cell division initiation in sporogenic aerial hyphae (diameter, 0.7 μm) (middle row). (Bottom row) At the time of Z-ring formation, SsgA and SsgB do not colocalize (diameter of aerial hyphae, 0.8 μm). For an explanation of stages (roman numerals between brackets), refer to the model in Figure 9. Bar, 1 μm.