Figure 3. Latent lymphoid potential in the GMP.

(A) Limiting dilution analysis of GMP and LMPP for T cell, B cell and myeloid differentiation potential. Cells were sorted at the indicated doses and co-cultured with OP9-DL1 for 14–18 days and with OP9 for 8–11 days under conditions that promote T cell, B cell and myeloid differentiation before FACS analysis for lineage markers. Frequencies of T cell, B cell differentiation were calculated using Pöisson statistics. Analysis of combined data from four independent experiments is shown. R² values are provided for each progenitor analysis. (B) 2000 LMPP (white circle), 7,500 GMP (a-grey diamond) or 30,000 GMP (b-black diamond,) were intravenously injected into sublethally irradiated recipient mice. Total and lineage- specific (Mac-1⁺, B220⁺, Thy1.2⁺) donor contribution (GFP⁺) was measured at days 5, 7, 13 and 22 post-injection. For every time point 2–4 mice per group were analyzed. (C) Representative LMPP and GMP donor contributions in the myeloid and B cell lineage (Mac-1⁺ vs. B220⁺) in the bone marrow at day 7 post-transplantation. LMPP and GMP contributions (GFP⁺Thy1.2⁺) to CD4 vs. CD8 profiles in the thymus is also shown at day 13 post-transplantation.