FIGURE 3.

Effect of rotenone on fatty acid oxidation and glucose uptake. A, effect of different concentrations of rotenone on metformin-induced palmitate oxidation in L6 myotubes. L6 cells were incubated with 120 μm palmitic acid with or without different concentrations of rotenone and 10 mm metformin for 3 h, then palmitate oxidation was measured as described under “Experimental Procedures.” Control (dark bar) or with metformin (light bar); *, p < 0.05. B, effect of rotenone on glucose uptake in L6 cells. L6 cells were incubated with 5 mm glucose with or without 250 μm rotenone. Data are expressed as per mg of cellular protein.