FIGURE 7.

Fc rNRP-1 drives angiogenesis in a fibroblast-HDMEC co-culture. A, HDMEC cells were grown for 6 days on a monolayer of confluent human dermal fibroblasts in the presence of VEGF-A₁₆₅ (30 ng/ml), Fc and Fc rNRP-1 (10 nm), and shNRP-1 (20 nm) (white bar indicates 100 μm). B, tube-like structures were photographed after staining for CD31 and analyzed as described under “Experimental Procedures.” The relative values depicting tubular morphogenesis were normalized versus the control cells. Significant differences between the tested agonists and the control were determined by Student's t test. Single asterisk (*) indicates that the p value is <0.01 for comparison to control.