FIGURE 10.

Effect of Bryo-1 administration in vivo on DC subpopulations in spleen. C57BL/6 mice received intraperitoneal injection of vehicle or Bryo-1, and 24 h later, the spleen cells were analyzed for the expression of CD11c, CD11b, or CD4 using flow cytometry. A and B show double staining for CD11c and CD11b, and CD11c and CD4, respectively. C depicts the total cellularity of the CD11c⁺CD11b⁺ and CD11c⁺CD4⁺ DCs in the spleens of vehicle or Bryo-1-treated C57BL/6 mice. The data represent means ± S.E. of three mice/group. The total number of cells in the Bryo-1-treated mice were significantly higher than the vehicle groups (p < 0.05).