FIGURE 3.

Bryo-1-induced activation of NF-κB is TLR4-mediated. BMDCs from C57BL/6 or TLR4^−/− mice were treated with Bryo-1 (10 ng/ml) or LPS (100 ng/ml). At the indicated time points, NF-κB DNA binding activity in nuclear extracts was determined by EMSA. Activated complexes were examined by autoradiography. Unt., untreated.