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Bryo-1 stimulation of BMDCs induces nuclear binding of IRF-1 to the promoter of RANTES gene. Nuclear extracts were prepared 2 and 4 h after Bryo-1 or LPS (4 h) treatment, and the presence of RANTES-IRF-1 binding activity was determined by EMSA. Complex formations (supershifted (SS) band) following incubation with anti-IRF-1, anti-IRF-3, and anti-IRF-7 were visualized by autoradiography and are indicated by arrows. FP, free probe.
