FIGURE 5.

Effect of NF-κB inhibitors on Bryo-1-induced NF-κB binding activity. BMDCs were incubated for 2 h with the NF-κB inhibitors, ALL (10 μm), MG132 (1 μm), and NBD peptide (200 μm), prior to Bryo-1 stimulation for 4 h and the NF-κB binding activity in the nuclear extracts was examined by EMSA and detected by autoradiography (A). The supernatants were assayed for the presence of RANTES by a sandwich ELISA (B). Vertical bars in B represent mean ± S.E. of three independent experiments, and asterisks represent statistically significant (p < 0.05) suppression of RANTES expression in the presence of NF-κB inhibitors. Unt, untreated.