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Induction of DNA binding activity and IFN-β production by Bryo-1 stimulation of BMDCs. Nuclear extracts were isolated from wild-type C57BL/6 or TLR4−/− mice treated with either Bryo-1 (10 ng/ml) or LPS (100 ng/ml) for the indicated times and subjected to EMSA using a 32P-labeled IFN-stimulated response element consensus sequence of the IFN-β gene promoter as probe. Activated complexes were detected by autoradiography. FP, free probe.
