FIGURE 3.

Inhibition of 6CF uptake by antivirals in oat1 knock-out adult kidney slices (function of the OAT3 transporter). Fluorescent photomicrographs show 6CF (1 μm) uptake (green) in adult oat1 KO kidney slices in the presence of a vehicle control (A), 2 mm probenecid (B), 100 μm zidovudine (C), 200 μm acyclovir (D), 100 μm tenofovir (E), 200 μm lamivudine (F), and 1 mm stavudine (G). Images are representative of triplicate slices from the same experiment. The bar graph (H) shows relative fluorescent signal strength derived from quantitative image analysis of 6CF in adult oat1 KO kidney slices. Scale bar = 0.2 mm.