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CID fragmentation spectra of peptide(245–258) (ELATGELLLDEI). The almost complete b and y series obtained from a non-noisy spectrum (35 fragment ions using 42 most intense peaks) gave a Mascot ion score of 101. This spectrum assignment indicates the break on the peptide bond after an isoleucine, which cannot be explained enzymatically but by the transformation of a monoiodo (or diiodo) tyrosine into a pyruvic acid residue.
