FIGURE 3.

Binding of non-template oligos containing the −10 promoter element consensus sequence to free σ⁷⁰ and holo-RNAP. A, sequences of oligos −12/+2, −12/−3, −12/−3, 7C, and −12/−6 are shown with the −10 promoter element sequence in larger size font. B, titration of free (211Cys-TMR) σ⁷⁰ with −12/+2, −12/−3, −12/−6, and −12/−3; 7C oligos. C, titration of (211Cys-TMR) σ⁷⁰ holoenzyme with −12/+2, −12/−3, and −12/+2; 7C oligos. D, titration of (211Cys-TMR) σ⁷⁰ holoenzyme with −12/−6 and −12/−6; 7C oligos. For all data panels, the solid lines correspond to a nonlinear regression fit of the data to Equation 1 (see “Experimental Procedures”).