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Binding of fork junction DNA probes to the β′-σ70 complex. A, sequence of the fork junction probes with the consensus extended −10 and −35 promoter element sequences in larger size font. B, titration of the β′-(211Cys-TMR) σ70 complex with the fork junction probes. The solid lines correspond to a nonlinear regression fit of the data to Equation 1.
