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Dissociation constants for the binding of −10 oligos to free σ70, σ70 RNAP holoenzyme, and the (β′-σ70) complex
The Kd values presented are averages from 2 to 3 individual experiments, the error is ±15%.
| Oligo | Kd, free σ | Kd holo-RNAP | Kd (β′-σ) | Kd (σ)/Kd (holo) | Kd (σ)/Kd (β′-σ) |
|---|---|---|---|---|---|
| μm | μm | μm | |||
| −12/+2 | 41 | 0.15 | 5.1 | 270 | 8 |
| −12/−3 | 43 | 1.5 | 8.7 | 29 | 4.9 |
| −12/−6 | 220 | 80 | 110 | 2.8 | 2 |
| lacUV5, −18 + 1 | 37 | 0.4 | 93 |
