FIGURE 2.

Cleavage of PR mRNA substrate pr39 by human RNase P in the presence of different EGSs. No EGS was added to the reaction mixture in lane 4. 10 (lanes 2 and 3) and 5 nm EGS (lane 1) were incubated with ³²P-labeled pr39 RNA substrate (10 nm) and either 5 (lanes 2–4) or 1 unit (lane 1) of human RNase P at 37 °C in a volume of 10 μl for 45 min in buffer A (50 mm Tris, pH 7.0, 100 mm NH₄Cl, 10 mm MgCl₂). Experimental details can be found under “Experimental Procedures.”