TABLE 2.
Levels of inhibition of viral gene expression in the cells expressing EGS PR-C125, PR-C125-C, PR-SER, PR-SER-C, and TK112, as compared to parental U373MG cells that did not express an EGS (U373MG)
The values are the means derived from triplicate experiments and values for the standard deviation that were less than 5% are not shown.
| Viral gene class | EGS RNA |
||||||
|---|---|---|---|---|---|---|---|
| U373-MG | TK112 | PR-SER-C | PR-C125-C | PR-SER | PR-C125 | ||
| IE1 mRNA | α | 0% | 0% | 1% | 0% | 1% | 0% |
| US2 mRNA | β | 0% | 0% | 0% | 1% | 0% | 2% |
| PR mRNA | γ | 0% | 1% | 7% | 8% | 80 ± 7% | 95 ± 9% |
| IE2 protein | α | 0% | 0% | 0% | 1% | 1% | 2% |
| UL44 protein | β,γ | 0% | 0% | 2% | 1% | 2% | 2% |
| PR protein | γ | 0% | 0% | 7% | 6% | 79 ± 7% | 95 ± 7% |
| gB | γ | 0% | 0% | 0% | 1% | 2% | 0% |
| gH | γ | 0% | 0% | 0% | 1% | 0% | 0% |