FIGURE 6.

Mitochondrial inheritance function of WT and mutant Gem1 proteins. A and B, corresponding differential interference contrast and digital fluorescence images of a wild-type strain with normal mitochondrial inheritance. C–F, gem1Δmmr1Δ cells with reduced mitochondrial inheritance. G–J, gem1Δmmr1Δ cells with defective mitochondrial inheritance. Cells are labeled with a mitochondria-targeted form of GFP (mito-GFP) and exhibit aberrant mitochondrial morphology in gem1Δmmr1Δ (21). K, quantification of mitochondrial inheritance by medium and large buds in gem1Δmmr1Δ (black bars). The presence of any mito-GFP in the bud was scored as successful inheritance. n ≥ 100. Error bars, S.D. values from three independent experiments. Bar, 5 μm.