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Nucleotide binding affinities of WT Gem1p(1–616) and other GTPases by FRET
The Kd values for dynamins were derived from tetramer models.
| GTPase | Nucleotide | Kd | Reference |
|---|---|---|---|
| μm | |||
| WT Gem1p(1–616) | Mant-GDP | 0.27 ± 0.10 | This study |
| GDP | 0.78 ± 0.09 | This study | |
| GTP | 0.63 ± 0.06 | This study | |
| YihA (E. coli) | GDP | 2.7 | Ref. 29 |
| Ffh (E. coli) | GDP | 1.3 | Ref. 43 |
| GTP | 1.2 | Ref. 43 | |
| Dynamin-1 (human) | GDP | 7.4 | Ref. 44 |
| GTP | 5.4 | Ref. 44 | |
| Dynamin-2 (rat) | GDP | 7.1 | Ref. 44 |
| GTP | 13.2 | Ref. 44 |
