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. 2010 Nov 1;286(1):380–390. doi: 10.1074/jbc.M110.184754

FIGURE 1.

FIGURE 1.

FABP3 is expressed in brown fat and induced by cold exposure. A, Fabp3 and Fabp4 mRNA levels in mouse tissues determined by real time PCR. cDNA was prepared from C57BL/6J mice, fasted overnight (n = 3). Values shown are in arbitrary units, normalized to housekeeping genes. B, FABP3 protein concentrations in the same samples as in A were determined by immunoassay. Values are expressed as nanograms of FABP3 per μg of total cellular protein. C, Fabp3 and Fabp4 mRNA levels in BAT, heart, skeletal muscle (quadriceps), and white adipose tissue at room temperature (RT) and after 4 h at 4 °C (n = 3). D, Fabp5 mRNA levels in BAT, heart, and skeletal muscle (quadriceps) at room temperature and after 4 h at 4 °C (n = 3). E, FABP3 protein concentration in BAT, heart, and skeletal muscle at room temperature and after 4 h at 4 °C. Protein extracts were obtained from the same mice as in C. In all panels, values represent the mean ± S.D. Sk, skeletal. *, p < 0.05 versus room temperature values.