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. 2010 Nov 1;286(1):380–390. doi: 10.1074/jbc.M110.184754

FIGURE 7.

FIGURE 7.

FABP3 levels are a determinant of fatty acid oxidation in a brown adipocyte cell line. A, mRNA levels for Ucp1 and Fabp3 in differentiated brown adipocytes were determined by real time RT-PCR. Cells were treated overnight with 1 μm norepinephrine (NE), 2 μm rosiglitazone (Rosi), or a combination of the two. Values represent mean ± S.D. (n = 3). B, OCR in differentiated brown adipocytes with and without overnight treatment with 1 μm norepinephrine was measured with the XF24 extracellular flux analyzer. The vertical dashed line indicates the addition of 200 μm palmitate. Values represent mean ± S.E. (n = 10). C, modulation of Fabp3 expression levels in differentiated brown adipocytes after transfection with an overexpression vector (left) or administration of RNAi (right). Values represent mean ± S.E. (n = 3). D, oxygen consumption rate in differentiated brown adipocytes after Fabp3 overexpression or siRNA knockdown. Cells were pretreated with 1 μm norepinephrine for 3 h before fatty acid oxidation rates were measured with the XF24 extracellular flux analyzer. Values represent mean ± S.E. (n = 10). *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus controls.