FIGURE 6.

H-89 inhibition of TPA-induced nucleosomal response and chromatin remodeler/modifier recruitment to Fosl1 regulatory regions in Hras1-transformed mouse fibroblasts. Serum-starved Ciras-3 cells were pretreated or not with H-89 prior to TPA stimulation for 0, 15, or 30 min. Formaldehyde-cross-linked mononucleosomes were prepared and used in ChIP assays with antibodies as indicated. Equal amounts of input and immunoprecipitated DNA were quantified by real-time quantitative PCR. Enrichment values are the mean of three independent experiments, and error bars represent S.D. H3ac, acetylated histone H3.