FIGURE 3.

Identification of candidate target genes degraded by miR-210 in KYSE-170 cells. A, Venn diagram showing overlapping sets of genes whose expression was decreased >5-fold by transfection of miR-210 and computationally predicted target genes of miR-210. B, list of four potential miR-210 target genes with a signal value of more than 50 in ncRNA-transfected cells upon microarray analysis. C–F, expression levels of the four potential miR-210 target mRNAs assessed by qRT-PCR. The values are shown relative to the value obtained with ncRNA (n = 3; *, p < 0.01). G, Western blot analyses of FGFRL1, NDUFA4, GPR177, LRP5L, and β-actin proteins.