FIGURE 7.

Differential localization of enolase in OPB knock-out versus wild type Leishmania. Wild type and OPB(−/−) Leishmania were fractionated by differential lysis. Western blots for enolase were performed on these cell extracts. Cell fractions were as follows: cell surface fraction (A), cytoplasmic fraction (B), nuclear fraction (C), and cytoskeletal fraction (D). Equivalent levels of enolase were detected in the cytoplasmic and nuclear fractions of both wild type and OBB −/− parasite, and it was not detected in the cytoskeletal fractions. Enolase was found to have accumulated on the cell surface of OPB(−/−) Leishmania but was not present on the cell surface of the wild type parasites.