FIGURE 8.

Gene expression of macrophages infected by OPB knock-out parasites. Murine bone marrow-derived macrophages were infected with either wild type or OPB(−/−) parasites for 0, 2, 6, 12, and 24 h. Gene expression from these macrophages was analyzed by microarray. A and B, array data for wild type (A) and OPB(−/−) (B) infections were analyzed to find statistically significant changes in gene expression compared with uninfected macrophages using a false discovery rate threshold of <1.0% and a minimum -fold change of 2. The x axis indicates the expression level of a given gene in the uninfected control macrophages. The y axis indicates the expression level observed in the infected macrophages. Genes with a significant change in expression after Leishmania infection are colored red for up-regulation and green for down-regulation. In the wild type infections, only 23 genes were differentially expressed (false discovery rate = 0.00%) compared with uninfected cells; however, in the OPB(−/−) infections, there were 459 significantly up-regulated genes (false discovery rate = 0.67%). C, heat maps of the array data were clustered to illustrate the differences between the different time courses. The OPB knock-out-infected macrophage arrays show a significant increase in gene expression (red) compared with the uninfected and wild type Leishmania-infected macrophage arrays.