FIGURE 5.

Phenylephrine treatment increases endogenous GLUT4 mRNA levels in preadipocytes with and without transactivating factors. A, 50 μg of COS7 whole cell extract transiently transfected with human HDAC5 or 120 μg of nuclear extracts of 3T3-L1 preadipocytes treated with or without phenylephrine for the indicated time periods and analyzed by SDS-PAGE and immunoblotting for HDAC5. The arrow indicates the expected appearance of HDAC5. Con, control; IB, immunoblot. B, 3T3-L1 preadipocytes were transiently transfected with transactivating plasmids encoding GEF, MEF2A, and LXRα. +, inclusion in transfection. The cells were treated with 10 μm phenylephrine for 24 h, and the RNA was isolated and analyzed by RT-PCR. The means ± S.E. from at least three independent experiments are shown; the data were analyzed by a Student's t test.